The method for the development and validation of First order derivative spectrometric method was developed for the simultaneous estimation of Budesonide and Salbutamol sulphate in their combined dosage form was developed. For this both the drugs were determined based on the derivative spectroscopic method at their respective zero crossing point (ZCP). The first order derivative spectra were obtained in methanol and determinations were made at 243 nm (ZCP of Budesonide) for Salbutamol sulphate and at 251 nm (ZCP of Salbutamol sulphate) for Budesonide. The linearity was obtained in the concentration range of 12-28 μg/ml for Budesonide and 30-70 μg/ml for Salbutamol sulphate. The mean recovery was 99.13±0.01 for Budesonide and 99.8±0.005 for Salbutamol sulphate and LOD and LOQ values for Budesonide were 0.214 μg/ml and 0.514 μg/ml respectively and for Salbutamol sulphate were 0.564 μg/ml and 2.3 μg/ml respectively. The recovery studies confirmed accuracy of proposed methods and low values of standard deviation confirmed precision of method. Thus a simple, sensitive, rapid, accurate, precise and economical first order derivative spectrometric method has been developed for the simultaneous estimation of Budesonide and Salbutamol sulphate.
Loading....